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KMID : 0043320080310050598
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Archives of Pharmacal Research
2008 Volume.31 No. 5 p.598 ~ p.605
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Lipoxygenase Inhibitory Constituents from Rhubarb
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Ngoc Tran Minh
Minh Pham Thi Hong
Hung Tran Manh
Thuong Phuong Thien
Lee Ik-Soo
Min Byung-Sun
Bae Ki-Hwan
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Abstract
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Phytochemical study on the ethanol extract of rhubarb led to the isolation of fifteen compounds, including five anthraquinones: chrysophanol (1), physcion (2), emodin (7), chrysophanol-8-O--D-glucopyranoside (9) and emodin-8-O--D-glucopyranoside (15), and ten stilbenes: desoxyrhaponticin (3), rhaponticin (4), resveratrol (5), desoxyrhapotigenin (6), rhapontigenin (8), piceatannol-3’-O--D-glucopyranoside (10), piceid (11), -viniferin (12), ampelopsin B (13) and isorhaponticin (14). Their structures were identified by comparing the physicochemical data with those of published papers. Among the isolated compounds, stilbene derivatives (3-6, 8 and 10-14) showed remarkable inhibitory effect on lipoxygenase with values ranging from 6.7 to . The inhibition kinetics analyzed by Lineweaver-Burk plots found that they were competitive inhibitors with the linoleic acid at the active site of lipoxygenase. In addition, stilbenes exhibited significantly free radical scavenging activity against with trolox equivalent activity capacity (TEAC) values ranging from 1.16 to 4.64. Whereas, anthraquinone derivatives (1-2, 7, 9 and 15) neither inhibited lipoxygenase nor scavenged free radical . These results indicated that stilbene derivatives were considerate to be mainly lipoxygenase inhibitor and free radical scavenger constituents of rhubarb.
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KEYWORD
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Rhubarb, Anthraquinone, Stilbene, Lipoxygenase, ABTS
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